Describe the relationship in between substrate concentration and also the initial reaction rate of one enzyme-catalyzed reaction. Is this a linear relationship? What happens to the early stage reaction price as substrate concentration increases?

Answer: Yes, the partnership is linear. The early stage reaction rate boosts as the substrate concentration increases. With more substrates, there will be a an ext of the reaction emerging until saturation started in 4 g, after ~ this concentration the reaction rate is steady( in all pH) as point out in graph and table.

You are watching: What is the maximum initial reaction rate for the lactase enzyme at ph 7?

What is the maximum initial reaction price for the lactase enzyme at pH 7?

Answer: 350

Explain why the preferably initial reaction rate cannot be got to at low lactose concentrations.

Answer: over there is not enough substrate for it to reach the maximun early rate.The maximun early stage reaction rate is350 (x10^6) molecules of product and there are enough substrates easily accessible to develop that many products.

What does her data indicate about the optimum pH level for this lactase-catalyzed reaction?

Answer: The day indicates the the highest possible enzyme task is in pH that 7. Saturation started in 4 g, after ~ this concentration the reaction rate is stable (in all pH) as mention in graph and table.

Enzymes duty most efficiently at the temperature the a usual cell, i m sorry is 37 levels Celsius. Boosts or reduce in temperature can considerably lower the reaction rate. What walk this suggest around the prominence of temperature-regulating mechanisms in organisms? Explain.

Answer: This mechanism is related to the homeostasis and the temperature regulation. Also, enzyme have perfect temperature the they duty at. Molecules relocating at high temperatures (more energy), and also reactions are much more likely come occur. Once temperature increases, it’s much more likely for reactions to occur. However, proteins can end up being denature once temperature are too high. If the temperature was to be raised or lessened in the enzymes region from this optimal temperature, climate the enzymes performance to catalyze reactions would decrease. Together enzymes function are crucial for organisms, the organisms have failure in their tasks and possibly death in enzymatic dysfunction. So, temperature is an extremely important in regulation mechanisms in organisms.

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People v a lactose intolerance room able to take assets such together Lactaid that contain the lactase enzyme v their meals. These products can it is in taken in pill form. Considering the truth that the pill type of the enzyme would have to travel with the person’s stomach, what special consideration would the producer of this product must be came to about?Answer: Lactase enzyme functions in non-acidic environment; the duodenum (the little intestine). So, when we require the lactase enzyme, it must be caused in the little intestine and the pill does not open in the stomach. We have the right to use some micro-coated pill to protect against the effect of stomach mountain on the enzyme and also release the enzyme in the stomach (pills). Also, there room different assets which usage in patient who experienced lactase deficiency save on computer lactase such together Lactase chewable tablets, Lactacid, Lacteeze. In addition, probiotics can use in this disorder. Yogurt and other conventional starter cultures and also probiotic bacteria in fermented and also unfermented milk products improve lactose digestion and also eliminate symptom of intolerance in lactose mal-digesters. These beneficial effects are because of microbial β-galactosidase in the (fermented) milk product, delay gastrointestinal transit, positive effects on intestinal functions and also colonic microflora, and also reduced sensitivity come symptoms. Undamaged bacterial cell walls, i m sorry act as a mechanical protection of lactase during gastric transit, and also the relax of the enzyme right into the little intestine are components of efficiency. Lactobacillus acidophilus supplementation may help in breaking down lactose and used in this disorder.
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Assignment 2